MitiMetCattle is scientific research project and belongs to program “IDEAS” which is founded by Science Fund of the Republic of Serbia in the group of Biomedical sciences. The main problem that the project is trying to solve is methane pollution from the enteric fermentation of dairy cattle through nutritional modulation of cows’metabolism. Namely, metan as greenhouse gas (GHG) contribute significantly to global warming which may provoke irreversible environmental changes. Compared to other livestock subsectors, cattle industry has major contribution to methane production with GHG effect. Despite the significant impact of cattle industry on CH₄ production, there is still no widely accepted strategy for reducing CH₄ emissions from cattle farms that would meet all requirements (significant reduction of GHG effect, economic justification, and beneficial impact on productivity). Also, farmers’ awareness of this problem is still low. The main aim of the project is to develop a methodology for an integrative approach to the analysis of rumen and overall metabolism, methane emission and milk yield, including molecular mechanisms that are crucial for metabolic turnover to greater milk production with reduced methane emission. In the frame of the project four innovative nutrition strategies for CH4 mitigation will be evaluated using developed methodology and statistical tool for prediction of methane emission per milk unit using easily detectable blood variables will be developed.